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MarinaBioLab TOTAL NUCLEIC ACID ISOLATION KIT

Fast, high-throughput purification of high-quality total nucleic acids including DNA and RNA from a wide variety of sample types.

The MarinaBioLab Total Nucleic Acid Isolation Kit delivers a complete system for the rapid isolation of total nucleic acids (DNA and RNA) from various sample types including viruses, bacteria, blood, and cells. The kit combines mechanical sample disruption using zirconia beads with an innovative magnetic bead-based purification technology, allowing high-throughput processing of up to 96 samples per run.

The MarinaBioLab kit isolates total nucleic acids of exceptional purity free from PCR inhibitors, making purified DNA and RNA ideal for sensitive downstream applications like PCR, real-time qPCR, reverse transcription PCR, next generation sequencing, and more.

Benefits

  • Fast, high-throughput nucleic acid purification in 1 hour
  • Bead-beating homogenization quickly breaks open tough samples
  • Magnetic beads ensure efficient binding and removal of nucleic acids
  • Scalable and automatable on KingFisherTM Duo Prime and Flex processors
  • Minimal sample-to-sample variation and hands-on time
  • Performance, 60 µg DNA recovery 

The MarinaBioLab Total Nucleic Acid Isolation Kit is highly effective across diverse sample types:

  • Clinical samples: Whole blood, plasma, serum, swabs, sputum
  • Bacteria and viruses: Bacterial cultures, body fluids, enriched samples
  • Environmental samples: Soil, water, manure, agricultural materials
  • Food samples: Dairy, produce, meat
  • Consistent high yields (60 µg) and purity rivaling competitors allow purified nucleic acids to work exceptionally in PCR, qPCR, RT-PCR, and next-gen sequencing.

Kit Contents

The MarinaBioLab Total Nucleic Acid Isolation Kit contains optimized reagent mixes and enzymes for 100 reactions:

  • Lysis/Binding Solution (guanidinium thiocyanate)
  • Total Nucleic Acid Magnetic Beads
  • Wash Solutions 1 and 2
  • Elution Buffer
  • Proteinase K
  • Zirconia beads
  • Required plates, strips, seals and comb
  • Simple Protocol

Disrupt sample by bead beating in Lysis/Binding Solution

Bind nucleic acids to magnetic beads

Wash beads to remove contaminants

Elute purified DNA/RNA

 

 

Specifications

Format

Compatible with 96-well plates, strips, tips, and combs for KingFisherTM Flex processor, 12-well strips for KingFisherTM Duo Prime processor

Isolation Technology

Magnetic bead-based purification

Sample Types

Clinical: Whole blood, plasma, serum, swabs, sputum Bacterial: Bacterial cultures 
Viral: Body fluids, enriched samples Environmental: Soil, water, manure, agricultural materials
Food: Dairy, produce, meat

Final Product

Total nucleic acids (DNA and RNA)

Applications

PCR, qPCR, RT-PCR, sequencing, microarrays, Southern blotting, northern blotting, library preparation

Throughput

High-throughput compatible; processes up to 96 samples per run

Reactions

100 purifications

Target

Total DNA and RNA

Processing Time

~1 hour hands-on time

Yield

Up to 100% recovery depending on sample type

Starting Material

Up to 2 mL liquid sample 
Up to 0.3 g solid sample

Store the entire MarinaBioLab Total Nucleic Acid Isolation Kit at 15°C to 25°C upon arrival as stated in the Instructions for Use section “Kit Components”.
All kit components including:

  • Lysis/Binding Solution
  • Magnetic Beads
  • Wash Solutions 1 and 2
  • Elution Buffer
  • Proteinase K
  • Plates, strips, seals, tips, combs

Should be stored at 15°C to 25°C.
Properly stored reagents are stable until the expiration date printed on the kit box and individual components. For optimal performance, store the kit at 15°C to 25°C immediately upon arrival. Avoid prolonged exposure to direct light.
Do not use expired or incorrectly stored reagents.

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